Oocyte (egg) Donation
Oocyte donation is the procedure in which a doner woman donates eggs. Those eggs are fertilized with the sperms of infertility patient’s husband & embryos are transferred in infertility patient’s uterus.
It is the only treatment available for infertility patients having Premature Ovarian Failure or age related ovarian failure.
When should this treatment be used?
It should be used for patients suffering from below:
1-Turner’s syndrome – a chromosomal anomaly where female is missing X chromosome.
2-Premature Ovarian Failure.
3-Advanced maternal age.
4-Post Cancer treatment like Chemotherapy, Radiotherapy.
5-Genetic abnormality in previous children related with oocyte dysfunction like Fragile X syndrome
6-Repeated implantation failures due to bad quality oocytes with normal semen parameters.
7-Repeated abortions due to bad quality of oocytes
How the oocyte(egg) donor is selected ?
While selecting a doner, thorough checks are done to ensure that doner and her oocytes are of best quality. At Akruti IVF, we ensure doner meets following criteria:
– Oocyte donor clear psychological & medical screening.
– Medical history shows no chronic illnesses.
– Physical Examination is clear
– Ovarian reserve assessment by ultrasonography & blood investigations shows healthy oocytes in good quantity.
– Should be between 21-32 years.
– All routine blood investigations rule out anaemia, diabetes, hypothyroidism or any other medical problems related with kidney & Liver.
– No history of any abnormal child due to chromosomal or genetic factor.
– There should not be too many or too frequent egg donation cycles.
– No history of any previous complications after egg retrieval procedure.
All the above assessments should be clear to ensure that donor’s eggs are of good quality.
Egg (oocyte) donation process
1 – Selection of donor & assignment to the recipient.
2 – In case of frozen oocytes, selection of oocytes, which are fulfilling the matching characteristics of recipient.
3 – Ovarian stimulation of donor.
4 – Follicular puncture & oocyte pick up of donor.
5 – Preparation of recipient’s endometrium.
6 – Invitro fertilization & embryo formation or oocyte thawing and Embryo formation in case of frozen oocytes.
7 – Embryo Transfer.
8 – Pregnancy test.
In some cases Embryos are frozen & then transferred after thawing where Pre-Implantation Genetic Screening (PGS) is required.
Egg (oocyte) donor cost & sources
Cost depends on following factors:
– The quantity of oocytes taken by patient. (Doner charges)
– Whether frozen or fresh oocytes are taken.
– Costs for psychological screening and genetic tests of doner.
– Cost of hormonal injections cost, commercial agency etc
What is the success criteria ?
Success rate depends on factors like:
– Age of donor
– Oocyte pick up procedure
– Husband’s sperm quality
– Recipient’s age & overall health
– Endometrium quality of recipient like thickness & blood supply
The procedure of oocyte donation is also known as third party reproduction.
Recipient should take proper counselling to explore emotional concerns.
Embryo Donation
In certain medical conditions, when couple’s own embryos are unable to give them pregnancy, they can opt for embryos through donation program.
When should one go for Embryo Donation?
♦ Repeated IVF failure.
♦ Chromosomal defects in embryos.
♦ Post menopausal patient with bad quality sperms of husband.
♦ Bad quality of self embryo of couple.
The procedure of this treatment
- A matching embryo is selected based on patient preference of features like skin color, hair color, cornea color, height of couple.
- Endometrial preparation is done by estrogen & progesterone combination.
- The endometrial phase is matched with embryo staging.
- As endometrium becomes ready, embryos are thawed (brought to normal temperature) & inseminated in the endometrial cavity.
- Hormonal support is given after procedure for implantation of the embryo.
- After Embryo Transfer, the patient is kept for 2 hours.
- HCG level is done after 15 days for confirmation of pregnancy.
If you have any queries regarding this treatment, we welcome you to book an appointment for counseling and discuss your queries and expected results with the doctor.
Embryo Transfer
There are two types of embryo transfers done in IVF cycle:
Fresh Embryo Transfer – embryo transferred in the same cycle as oocyte pickup.
Frozen Embryo transfer – embryos are cryo preserved and transferred later when patient is ready.
Fresh Embryo Transfer
Fresh embryo transfer means that the transfer of embryo is done in the same cycle of oocyte pick up, on third or fifth day.
What are the advantages of fresh Embryo transfer?
1-Fresh embryo transfer gives pregnancy in short duration as it is done in the same cycle as pick up.
2-Cost effective as no money is spend in freezing the embryos & thawing in next cycle till transfer is done.
3-There is no risk for embryos to get some growth problems after thawing which sometimes may occur due to technical error or human error.
Under what conditions it is helpful?
1-Poor responders who has limited embryo formations.
2-Normal responders with excellent quality of embryos with controlled hormone levels of progesterone.
3-good quality of endometrium with proper thickness & good blood supply with normal values of uterine blood supply studied with Colour Doppler.
4-Good quality corpus luteum studied with Colour Doppler.
All the above conditions favour fresh embryo transfer & give good success rate in implantation.
What are the contra indications for doing fresh embryo transfer?
1-When progesterone levels in blood are high, generally fresh embryo transfer is not done due to high rate of implantation failure.
2-In case of ovarian hyperstimulation syndrome which occurs due to multiple follicles formation in case of PCOD, fresh embryo transfer is not advisable as it can give serious health issues to patient.
3-When there is a possibility of genetic abnormalities and embryos need to be checked for that using Pre Implementation Genetic Screening (PGS) \ Pre Implementation Genetic Diagnostic (PGS) it takes time for test results to come. In such a case there is no option but to freeze the embryo till that time. Hence fresh embryo transfer is not possible in such cases.
4-Endometrum showing less thickness & poor blood supply, which does not favour implantation of embryo.
In all above conditions Frozen Embryo transfer need to be done to improve the success rate of conception.
Frozen Embryo Transfer
Frozen embryo transfer cycle is a procedure done to improve the success rate of pregnancy in certain conditions like PCOD and in few IVF cycles where clinician feels that patient stands a better chance of pregnancy with frozen embryo transfer as compared to fresh transfer of embryos. The Female infertility treatment thane also ensures zero chance of patient developing OHSS.
Procedure
♦ Thawing procedure is done on frozen embryos Using special kit called Thawing Kit.
♦ After thawing, embryos are kept in an incubator.
♦ Embryo transfer can be done after 1 hour of thawing.
♦ The endometrium is synchronized with embryo stage & then thawed embryos are inseminated. Thawed embryos can be of D3(89-10 cellar) OR Blastocyst stage (D5 embryo)
Indication for Frozen embryo transfer cycle
♦ Transfer of fresh embryo has failed.
♦ Transfer of fresh embryo has to be cancelled due to severe OHSS.
♦ Embryo transfer cancelled due to acute medical OR surgical illness
♦ Embryos require Pre-Genetic Screening
Conclusion
Whether to transfer fresh or frozen embryo? Decision is taken based on which method will give higher success rate in conception, without any side effect to the patient.
Transfer of Frozen embryo is undertaken only if patient’s body need to be made ready to take Fresh embryo. In case freezing of embryos is need, Akruti IVF has services for cryopreservation of embryos, to preserve them for the duration needed by the patient to be ready.
At Akruti IVF LLP the experienced doctors offer treatment best suited to your need. We has both surgical as well as non invasive procedures to treat Male and Female infertility. Patients of Thane, Kalyan Ambernath, Badalapur, Palava, Mumbra area would find it very convenient and accessible. Please call the number mentioned on top of this page to know how we can assist you.
Sperm Donation
Sperm donation is a process in which sperms are provided by a man with the intention of using in Artificial Insemination or other fertility treatment in women like IVF, ICSI who are not his sexual partners in order to give pregnancy to them.
Conditions in which Donor Sperms are required in infertility treatment?
1 – Males with azoospermia (no sperms found by Sperm Retrieval method).
2 – Normal females with fertilization failures due to poor quality sperms.
3 – Genetically abnormal embryos due to high DNA fragmentation which is medically non correctable.
4 – Male with infection like HIV have high risk of transferring to the baby & female patient.
5 – Low sperm count or abnormal sperms in which patient can’t afford ICSI cycle & female partner is normal.
Criteria for selection of Sperm Donor
· Complete medical history of donor is clear
· Physical examination
· Information about age, weight, any past surgeries, allergies to any medicines, previous personal history & family history of any disease.
· Height, hair colour, body colour, cornea colour, blood group should match with husband.
· Examination for any cardiac problem or Hypertension & Diabetes with no signs & symptoms
· Blood investigations for HB ,Blood Sugar, Bleeding Disorders, Liver function tests, Cholesterol, Kidney function test, blood tests for infection like AIDS & Hepatitis (HIV & HBSAg), Thyroid Function Test
· All the above tests should have been within past 6 monthly and the results should be good.
· Psychological evaluation for his mental fitness & details of outcome & about biological children
· Detailed Semen Examination for sperm count & motility for evaluation of fertilization power of sperms
· Genetic testing is done with history taking of cystic fibrosis in any close relation or History of any chromosomal disorder in family accordingly risk is calculated & if required tests are done
Steps for Sample collection
· Sperm Donation Process is done at Sperm Bank
· Donor is sent to clean & sterile private room with sterile container
· Donor has to masturbate & collect the sample
· Container is then handed over to responsible person or kept in safe & special environment
Post sample collection evaluation & Storage
· Sample is evaluated for count & motility
· Part of the sample is send for semen culture if it comes infected sample is discarded though it is frozen
· After evaluation if sample is normal it is cryopreserved or Frozen
· After 6 months blood tests of donor for infection are again evaluated
· If blood tests are normal sample can be used for ART procedures
What is the duration for storing sperm sample?
Good quality sperm sample can be stored for up to 20 years with deep freezing and almost forever with cryopreservation.
It does not damage the quality of sperms.
Are there any side effects of cryopreserved or frozen sperms?
Cryopreserved sperm does not have any negative side effects.
Properly screened sample is not having any increase in birth defects or increase in failure of conception.
Chances of getting any birth defects are equivalent to fresh sperm sample.
Conclusion
Sperm Donation is a very useful provision, which is legally approved, & can be used for the couples where husband’s sperms are not able to give pregnancy.
Properly selected sperm donor with above mentioned criteria gives the best results.
Fresh sample is never used for sperm donation. Sample provided by sperm donor is always frozen or cryopreserved for 6 months. They are used after retesting the patient for any infection. This is to ensure that diseases like HIV which take long duration to appear are not present in the patient.
Akruti IVF centre follows all the above criteria & has been very successful in giving healthy pregnancies by Sperm Donation. Listed below are couple of other procedures which may be needed by a patient taking Sperm Donation services:
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